Importance of the leader peptide sequence on the lanthipeptide secretion level

Lanthipeptides are ribosomally synthesized and posttranslationally modified peptides. Their precursor peptide comprises of an N‐terminal leader a C‐terminal core peptide. Here, the is crucial for enzyme recognition especially modification enzymes acts furthermore as secretion signal lanthipeptide exporter. The target site posttranslational modifications contains dehydrated amino acids lanthionine rings. Nisin produced by Gram‐positive bacterium Lactococcus lactis one best‐studied lanthipeptides used model system to study their processes. secreted we present in vivo analysis NisT absence machinery allowing mutants impact solely on activity NisT. Additionally, created hybrids provide new insights, how effected unnatural focus transporter alone enabled us determine within nisin.